RCA-free production of third generation adenoviral vectors

Construction of first-generation adenoviral vectors.

Genetically modified adenoviruses (Ads) make attractive vectors for the delivery of exogenous DNA to mammalian cells for basic science and gene therapy applications. Ad vector production consists of (1) cloning a trangene into an infectious plasmid by in vivo recombination in bacteria, (2) rescuing and propagating the vector in complementing cells, and (3) purifying the vector. All of this can ...

Production of Rca

Concrete pavement recycling is a relatively simple process that involves breaking, removing and crushing hardened concrete from an acceptable source to produce recycled concrete aggregate (RCA). The formal process of RCA production typically involves an initial evaluation of the existing source concrete; pavement preparation, which may include removal of an asphalt overlay; pavement breaking, r...

Adenoviral Vectors for Hemophilia Gene Therapy

Hemophilia is an inherited blood clotting disorder resulting from deficiency of blood coagulation factors. Current standard of care for hemophilia patients is frequent intravenous infusions of the missing coagulation factor. Gene therapy for hemophilia involves the introduction of a normal copy of the deficient coagulation factor gene thereby potentially offering a definitive cure for the bleed...

9 - Targeted Adenoviral Vectors I: Transductional Targeting

Efficient generation of recombinant adenoviral vectors by Cre-lox recombination in vitro.

BACKGROUND Although recombinant adenovirus vectors are attractive for use in gene expression studies and therapeutic applications, the construction of these vectors remains relatively time-consuming. We report here a strategy that simplifies the production of adenoviruses using the Cre-loxP system. MATERIALS AND METHODS Full-length recombinant adenovirus DNA was generated in vitro by Cre-medi...